Antibody productionOne Ahi1glutathione Stranferase (GST) fusion protein was constructed by inframe cloning of the Cterminal region of Ahi1 cDNA into a pGEX4T1 vector (amino acids 961 with a high homology to human AHI1 sequences). The Ahi1 fusion protein was then purified and injected into rabbits. Serum was collected and the titers of antibody were determined by enzymelinked immunisorbent assay (ELISA) and Western blot analysis.He did, though, have two rushing touchdowns and a receiving touchdown to go with four catches for 18 yards.Ponder had what is becoming his usual tough day behind center as he went 18 for28 for 236 yards with a touchdown and three interceptions, along with losing a fumble for the Vikings (01), who went 106 last season and lost in the first round of the playoffs."Tough loss for our football team on the road," said Minnesota head coach Leslie Frazier. "We didn't do things you have to do on the road to win. Turnovers on the road at home as well,