louis vuitton official website usa When you are sure colonization is complete you will need to break up the grain so that you can inoculate your other substrates. Using a tire is perfect for this. Just make sure there are no cracks in the jar or it could break. You should also use safety goggles when doing this. After the grains have broken up, place it back in your incubation area and wait 24 hours. This will help rule out contaminations. The next day, mycelium should be growing again. If it has not within 24 hours the jar is considered contaminated by bacteria and should be thrown out. If you use it then you are potentially contaminating all of your new substrate jars.

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